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1. 发明授权

US10988720B1 Peel plate assembly 有权
公开(公告)号：US10988720B1
公开(公告)日：2021-04-27
申请号：US14935692
申请日：2015-11-09
申请人： Richard Skiffington , Robert Markovsky , Byron D. Roberts , Stanley E. Charm , Benjamin Mathieu , Robert S. Salter
发明人： Richard Skiffington , Robert Markovsky , Byron D. Roberts , Stanley E. Charm , Benjamin Mathieu , Robert S. Salter
IPC分类号： C12M1/00 , C12M1/32 , C12M1/12
摘要： Peel plates and plate assemblies are shown and described. In one embodiment, a peel plate assembly includes a semi-rigid plate, a removable cover seal, and an adhesive to provide a moisture vapor barrier. In particular embodiments, the moisture vapor barrier provides less than about one pound peel adhesion and a stiffness for reapplication without wrinkling. The result is a peel plate, assembly, and method for the enumerating a microorganism, when present, in a sample.

2. 发明授权

US09008373B2 Device, system and method for transit testing of samples 有权
标题翻译：样品转运试验的装置，系统和方法
公开(公告)号：US09008373B2
公开(公告)日：2015-04-14
申请号：US13696445
申请日：2011-05-06
申请人： Robert J. Markovsky , Stanley E. Charm , Richard T. Skiffington
发明人： Robert J. Markovsky , Stanley E. Charm , Richard T. Skiffington
IPC分类号： G01N33/04
CPC分类号： G01N33/04
摘要： Disclosed herein is a method and device for testing an agricultural product for the presence of an unwanted residue including a reader-incubator combination that can utilize onboard motor vehicle systems, such as an onboard microprocessor and onboard power supply.
摘要翻译：本文公开了一种用于测试农产品以存在不想要的残留物的方法和装置，包括可以利用车载微处理器和车载电源的机载车辆系统的读取器 - 培养箱组合。

3. 发明申请

US20140271362A1 RECOGNITION TEST STRIPS 审中-公开
标题翻译：识别测试条
公开(公告)号：US20140271362A1
公开(公告)日：2014-09-18
申请号：US13796471
申请日：2013-03-12
申请人： Robert J. Markovsky , Stanley E. Charm , Paul E. Graham , Richard T. Skiffington
发明人： Robert J. Markovsky , Stanley E. Charm , Paul E. Graham , Richard T. Skiffington
IPC分类号： G01N21/55
CPC分类号： G01N21/8483 , G01N2021/8488
摘要： Test strips for the detection of at least one analyte in a sample are shown and described. In one embodiment, the test strip includes a test zone, a control zone and a surface reflectance profile to monitor the test strip continuously until the presence or absence of an analyte is detected. In other embodiments, a lateral flow assay includes a surface reflectance profile to monitor the test strip prior to the detection. The surface reflectance profile may include at least one flow reference area and/or at least one test result reference area. The result is systems and methods to improve the detection of the presence and/or absence of at least one analyte in a sample.
摘要翻译：显示和描述了用于检测样品中至少一种分析物的测试条。在一个实施例中，测试条包括测试区域，控制区域和表面反射率分布，以连续监测测试条，直到检测到分析物的存在或不存在。在其它实施例中，横向流量测定包括在检测之前监测测试条的表面反射率分布。表面反射率分布可以包括至少一个流动参考区域和/或至少一个测试结果参考区域。结果是改进对样品中至少一种分析物的存在和/或不存在的检测的系统和方法。

4. 发明申请

US20130177214A1 DEVICE, SYSTEM AND METHOD FOR TRANSIT TESTING OF SAMPLES 有权
标题翻译：装置，系统和方法用于样本的过渡测试
公开(公告)号：US20130177214A1
公开(公告)日：2013-07-11
申请号：US13696445
申请日：2011-05-06
申请人： Robert J. Markovsky , Stanley E. Charm , Richard T. Skiffington
发明人： Robert J. Markovsky , Stanley E. Charm , Richard T. Skiffington
IPC分类号： G01N33/04
CPC分类号： G01N33/04
摘要： Disclosed herein is a method and device for testing an agricultural product for the presence of an unwanted residue including a reader-incubator combination that can utilize onboard motor vehicle systems, such as an onboard microprocessor and onboard power supply?
摘要翻译：本文公开了一种用于测试农产品是否存在不想要的残渣的方法和装置，包括可以利用车载微处理器和车载电源的机载车辆系统的读取器 - 培养箱组合？

5. 发明授权

US06319466B1 Test device for detecting the presence of a residue analyte in a sample 失效
标题翻译：用于检测样品中残留物分析物的存在的测试装置
公开(公告)号：US06319466B1
公开(公告)日：2001-11-20
申请号：US09118135
申请日：1998-07-16
申请人： Robert J. Markovsky , Cheryl A. Boyer , Stanley E. Charm , Paul R. Donahue , Yael Agi Glickman , Steven J. Saul , Joan L. Scheemaker , Richard T. Skiffington , Shefali B. Trivedi , Eliezer Zomer
发明人： Robert J. Markovsky , Cheryl A. Boyer , Stanley E. Charm , Paul R. Donahue , Yael Agi Glickman , Steven J. Saul , Joan L. Scheemaker , Richard T. Skiffington , Shefali B. Trivedi , Eliezer Zomer
IPC分类号： G01N2178
CPC分类号： G01N33/558 , A23D9/00 , A23K10/30 , A23K20/158 , C11B3/001 , C11B7/00 , C11C1/04 , C11C3/10 , C11C3/123 , C12N9/0083 , C12N15/8216 , C12N15/8222 , C12N15/8234 , C12N15/8247 , G01N33/537 , G01N33/54366 , G01N2201/0221 , Y10S435/805 , Y10S435/97 , Y10T436/10 , Y10T436/105831
摘要： A test device for detecting the presence of a residue analyte in a sample includes a support strip and a sample-absorbing matrix attached to the support strip. The sample-absorbing matrix has a material for absorbing an amount of the sample. The test device also includes a mobile-phase support for holding a mobile-phase composition. The mobile-phase support is attached to the support strip and in contact with the sample-absorbing matrix. A mobile-phase composition is disposed on the mobile-phase support and has a receptor for binding with the analyte. The mobile-phase composition can be carried in the sample. A stationary-phase membrane is attached to the support strip and has a first membrane end in contact with the mobile-phase composition and a second membrane end. The membrane allows lateral capillary flow of the sample from the first membrane end to the second membrane end. A test zone is on the stationary-phase membrane between the first membrane end and second membrane end and having a first binder for binding with an unbound receptor. A control zone is the stationary-phase a membrane between the test zone and second membrane end and has a second binder for binding with an analyte-bound receptor or residual unbound receptor.
摘要翻译：用于检测样品中残留物分析物的存在的测试装置包括支撑条和连接到支撑条的样品吸收基质。样品吸收基质具有用于吸收一定量样品的材料。测试装置还包括用于保持流动相组成的流动相支持。流动相载体连接到支撑条上并与样品吸收基质接触。移动相组合物设置在流动相载体上并具有用于与分析物结合的受体。流动相组合物可以携带在样品中。固定相膜连接到支撑条上并具有与流动相组合物接触的第一膜端和第二膜端。膜允许样品从第一膜端到第二膜端的侧向毛细管流动。测试区位于第一膜端和第二膜端之间的固定相膜上，并具有用于与未结合受体结合的第一粘合剂。控制区是测试区和第二膜端之间的固定相膜，并且具有用于与分析物结合的受体或残留的未结合受体结合的第二粘合剂。

6. 发明授权

US5283180A Bioluminescence method for the determination of pesticides 失效
标题翻译：生物发光法测定农药
公开(公告)号：US5283180A
公开(公告)日：1994-02-01
申请号：US818782
申请日：1992-01-09
申请人： Eliezer Zomer , Steven Saul , Stanley E. Charm
发明人： Eliezer Zomer , Steven Saul , Stanley E. Charm
IPC分类号： C07D277/68 , C07D277/64 , C12Q1/66 , G01N21/76 , G01N33/567 , G01N33/60 , C12Q1/00
CPC分类号： G01N33/567 , C07D277/64 , C12Q1/66 , G01N33/60 , Y10S435/975
摘要： A method for the rapid and sensitive determination of organophosphate and carbamate pesticides is disclosed. The method employs an insect brain preparation having a mixture of receptors or enzymes with sites that interact with the organophosphate and carbamate pesticides. The pesticides alter or reduce brain activities of the insect brain preparation which are inversely correlated with pesticide concentration in a test sample. The activity is measured by employing substrates which upon exposure to the insect brain preparation are structurally altered and a light emission reaction is observed. The substrates used are 6-substituted D-Luciferin esters wherein the D-Luciferin ester in the presence of the insect brain preparation is inhibited during hydrolysis while in the presence of the pesticide to be determined. Further the D-Luciferin ester in the presence of the insect brain preparation reacts to cleave the substituted ester group at the 6-position to produce D-Luciferin for further reaction with ATP and luciferase to produce bioluminescence. The bioluminescence can then be measured and compared with a control in order to determine the concentration of a pesticide in the test sample.
摘要翻译：公开了一种用于快速和灵敏地测定有机磷酸盐和氨基甲酸酯农药的方法。该方法采用具有受体或酶的混合物的昆虫脑制剂与与有机磷酸盐和氨基甲酸酯农药相互作用的位点。农药改变或减少与测试样品中农药浓度成反比关系的昆虫脑制剂的脑活动。活性通过使用底物测量，其在暴露于昆虫脑制备物结构上改变并观察到发光反应。使用的底物是6-取代的D-萤光素酯，其中在昆虫脑制剂存在下的D-萤光素酯在水解期间在待测定的农药的存在下被抑制。此外，在昆虫脑制剂存在下的D-萤光素酯反应以在6位切割取代的酯基，以产生D-萤光素，以进一步与ATP和荧光素酶反应以产生生物发光。然后可以测量生物发光并与对照进行比较，以确定测试样品中农药的浓度。

7. 外观设计

USD302639S Milk sampling apparatus 失效
公开(公告)号：USD302639S
公开(公告)日：1989-08-08
申请号：US62221
申请日：1987-06-12
申请人： Stanley E. Charm
设计人： Stanley E. Charm

8. 发明授权

US4330626A Method of preparing high-activity, low-bacteria, urease enzyme 失效
标题翻译：制备高活性，低细菌，脲酶的方法
公开(公告)号：US4330626A
公开(公告)日：1982-05-18
申请号：US131742
申请日：1980-03-19
申请人： Henry E. Blair , Stanley E. Charm , Richard P. Crowley
发明人： Henry E. Blair , Stanley E. Charm , Richard P. Crowley
IPC分类号： C12N9/80 , C12N13/00
CPC分类号： C12N13/00 , C12N9/80
摘要： In a method of preparing the enzyme, urease, from jack beans, wherein the jack beans are reduced to a fine particle size and the fine particles so reduced constitute urease, the fine jack-bean particles thereafter irradiated for a period of time, the irradiated jack beans then recovered and employed as a source of urease enzyme, the improvement which comprises: irradiating the whole jack beans prior to any size reduction of the seeds of the jack beans, to provide a urease derived from the white seeds of the irradiated whole jack beans having an improved activity level.
摘要翻译：在制备来自千斤顶的酶的尿素酶的方法中，其中千斤顶豆被还原成细粒度，并且如此减少的微粒构成尿素酶，此后将细小的jack豆颗粒照射一段时间，照射千斤顶豆然后回收并用作脲酶的来源，其改进包括：在大豆种子的任何尺寸减小之前照射整个千斤顶豆，以提供衍生自经照射的整个杰克的白种子的脲酶豆类具有改善的活动水平。

9. 发明授权

US4238521A Process for the removal of antibiotics from milk 失效
标题翻译：从牛奶中去除抗生素的过程
公开(公告)号：US4238521A
公开(公告)日：1980-12-09
申请号：US76600
申请日：1979-09-18
申请人： Stanley E. Charm
发明人： Stanley E. Charm
IPC分类号： A23C7/04 , A23C9/15
CPC分类号： A23C7/043
摘要： A method of removal of penicillin from penicillin-contaminated milk, which method comprises contacting the contaminated milk with activated charcoal and recovering a penicillin-free milk product.
摘要翻译：从青霉素污染的牛奶中去除青霉素的方法，该方法包括使受污染的乳与活性炭接触并回收不含青霉素的乳制品。

10. 发明授权

US4102455A Radioassay kit for method of determining methotrexate 失效
标题翻译：用于测定甲氨蝶呤的方法的放射测定试剂盒
公开(公告)号：US4102455A
公开(公告)日：1978-07-25
申请号：US753852
申请日：1976-12-23
申请人： Stanley E. Charm , Henry E. Blair
发明人： Stanley E. Charm , Henry E. Blair
IPC分类号： G01N33/50 , C12Q1/32 , G01N33/94 , A61B19/00 , A61K43/00
CPC分类号： G01N33/94 , C12Q1/32 , Y10T436/147777 , Y10T436/174614
摘要： A radioassay system for the determination of methotrexate in biological fluids based on the competitive binding of labeled and unlabeled methotrexate to the enzyme dihydrofolate reductase. Samples of unknown methotrexate level are mixed with I.sup.125 labeled methotrexate. A portion of the total methotrexate present is bound by the addition of enzyme, and the unbound methotrexate is removed with charcoal. The level of bound I.sup.125 labeled methotrexate is measured in a gamma counter. To calculate the methotrexate level of the unknown samples, the displacement of bound labeled methotrexate caused by the unknowns is compared to the displacement caused by known methotrexate standards.
摘要翻译：基于标记和未标记的甲氨蝶呤与酶二氢叶酸还原酶的竞争性结合的用于测定生物流体中甲氨蝶呤的放射分析系统。未知甲氨蝶呤水平的样品与I125标记的甲氨蝶呤混合。甲氨蝶呤总量的一部分是通过添加酶结合的，未结合的甲氨蝶呤用木炭除去。在γ计数器中测量结合的I125标记的甲氨蝶呤的水平。为了计算未知样本的甲氨蝶呤水平，将由未知物引起的结合的标记的甲氨蝶呤的位移与由已知的甲氨蝶呤标准引起的位移进行比较。

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